Localization of P. falciparum SHMT isoforms. Parasites were transfected with pGFP (A), pRL PfcSHMT (B), pGL_PfmSHMT (C), and pGL_N24del PfmSHMT (D) plasmids expressing fluorescent signals from GFP or DsRed. Schematic diagrams of the recombinant plasmids used are shown alongside the confocal micrographs. Mitochondrion and nucleus is stained with Mitotracker™ (red) and Hoechst 33258 (blue) dye respectively. DIC, differential interference contrast image. The distribution of DsRed-tagged PfcSHMT appears to be dominantly in cytoplasm (B). The distribution of GFP-tagged PfmSHMT was colocalized with Mitotracker™ within the mitochondria (C). Co-localization of N-24 truncated-PfmSHMTGFP with Mitotracker™ (D), indicated that this 24 N-terminal amino acid sequence does not play a role as a mitochondria targeting signal.
Pornthanakasem W, Kongkasuriyachai D, Uthaipibull C, Yuthavong Y, Leartsakulpanich U. Plasmodium serine hydroxymethyltransferase: indispensability and display of distinct localization. Malar J. 2012 11:387.
Other associated proteins