Effect of anti-PfRH1 inhibitory mAbs on merozoite surface expression of EBA175 during invasion. Isolated T994 (a,c) were directly incubated with erythrocytes containing 4 mM Cyto D in the absence (Control) and presence (C41) of C41 (0.2mgml-1) before analysis of expression of microneme protein EBA175 and merozoite surface marker MSP1 at the junction. Both differential interference contrast (DIC) and fluorescence images were captured. Nuclear DNA was counterstained with DAPI (blue). EBA175 is shown in green, whereas MSP1 is shown in red. Fluorescence images, merged fluorescence images (DAPI and green or DAPI with red) and merged fluorescence images with DIC images are shown. Scale bars=10 mm.
Gao X, Gunalan K, Yap SS, Preiser PR. Triggers of key calcium signals during erythrocyte invasion by Plasmodium falciparum. Nat Commun. 2013;4:2862. PMID:
Other associated proteins
|PF3D7_0731500||erythrocyte binding antigen-175|