Stage-specific expression and subcellular localization of PfPCNA proteins. (B and C) IFA was performed on glass slides containing parasite smears, which were treated with antibodies against PfPCNA1 (B) or PfPCNA2 (C). PfPCNA1 (green) shows nuclear foci formation in replicating trophozoite stage and multinucleate schizont stage, whereas PfPCNA2 (red) shows both nuclear and cytoplasmic diffuse staining. DAPI (blue) stains the nuclei. The IFA showed distinct nuclear foci of endogenous PCNA1 during trophozoite and schizont stages of the parasites (B). Interestingly, endogenous PfPCNA2 shows a more diffused pattern throughout the nucleus and cytoplasm (C).

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