Phosphorylation of PfCDPK1 at S64 (phosphorylation at Serine 64) occurs during the schizont (Schiz) stage of parasite development. (e) A rendered image where deconvoluted z stacks were reconstructed in 3D, with interpolation, of wild-type parasites stained with DAPI (blue), the structural CDPK1 antibody to reveal the total pool of PfCDPK1 (red) and the CDPK1-pS64 antibody (green). (f) Same as e but in this case the images for z stacks are of a free merozoite. These results are representative of at least three experiments. Scale bars, e-1 mm, f-0.5 mm. Immunofluorescence using an anti-HA antibody that detected PfCDPK1-HA in transgenic parasites revealed the peripheral (membrane) localization of PfCDPK1 in the nascent merozoites contained within the late-stage schizonts or free merozoites. In contrast, S64-phosphorylated PfCDPK1 appeared as discrete ‘punctate spots’ at the periphery of merozoites within late Schizonts .
Alam MM, Solyakov L, Bottrill AR, Flueck C, Siddiqui FA, Singh S, Mistry S, Viskaduraki M, Lee K, Hopp CS, Chitnis CE, Doerig C, Moon RW, Green JL, Holder AA, Baker DA, Tobin AB. Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion. Nat Commun. 2015 6:7285