Deletion of the repeat region of REX1 decreases the number of Maurer's cleft puncta. A. Immunofluorescence microscopy of acetone-fixed infected RBCs probed with anti-SBP1 (red) and anti-REX1 (green). Nuclei are stained with DAPI (blue). Scale bar = 3 μm. Full-length REX1-GFP is correctly localized at the Maurer's clefts and that these transfectants exhibit an average number of puncta equivalent to that of the 3D7 parent. By contrast, the REX1Δ371-579-GFP parasites exhibited a striking Maurer’s cleft phenotype, with only 2 + 0.3 punctate structures observed in the infected RBC cytoplasm.
McHugh E, Batinovic S, Hanssen E, McMillan PJ, Kenny S, Griffin MD, Crawford S, Trenholme KR, Gardiner DL, Dixon MW, Tilley L. A repeat sequence domain of the ring-exported protein-1 of Plasmodium falciparum controls export machinery architecture and virulence protein trafficking. Mol Microbiol. 2015 Aug 24. [Epub ahead of print]
Other associated proteins
|PF3D7_0935900||ring-exported protein 1|