REX1(Δ371–579)-GFP parasites exhibit giant Maurer’s clefts. A. REX1(Δ371–579)-GFPinfected RBCs fixed with paraformaldehyde, permeabilized with EqtII and labelled with anti-GFP. B. REX1-GFP and REX1(Δ371–579)-GFP-infected RBCs were fixed with paraformaldehyde/glutaraldehyde and probed with anti-GFP (green) and anti-SBP1 (red). Samples were examined using 3D-SIM. 3D-SIM reveals giant Maurer's clefts with an apparently convoluted surfaceC. REX1(Δ371–579)-GFP-infected RBCs were labelled with BODIPY-ceramide, fixed with paraformaldehyde/ glutaraldehyde, labelled with DAPI and imaged using widefield deconvolution microscopy. The right hand panel shows rendering of the surface of the 3D structure using Imaris software. REX1Δ371-579-GFP appears to accumulate at discrete regions of the PVM as does EXP-2. D. REX1(Δ371–579)-GFP-infected RBCs were fixed with paraformaldehyde/ glutaraldehyde and probed with anti-EXP2 (green) and anti-REX1 (red). Samples were examined using deconvolution microscopy. Scale bars = 3 μm; zoom bar = 1 μm.

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