PF3D7_1471100 exported protein 2

REX1(Δ371–579)-GFP parasites exhibit giant Maurer’s clefts. A. REX1(Δ371–579)-GFPinfected RBCs fixed with paraformaldehyde, permeabilized with EqtII and labelled with anti-GFP. B. REX1-GFP and REX1(Δ371–579)-GFP-infected RBCs were fixed with paraformaldehyde/glutaraldehyde and probed with anti-GFP (green) and anti-SBP1 (red). Samples were examined using 3D-SIM. 3D-SIM reveals giant Maurer's clefts with an apparently convoluted surfaceC. REX1(Δ371–579)-GFP-infected RBCs were labelled with BODIPY-ceramide, fixed with paraformaldehyde/ glutaraldehyde, labelled with DAPI and imaged using widefield deconvolution microscopy. The right hand panel shows rendering of the surface of the 3D structure using Imaris software. REX1Δ371-579-GFP appears to accumulate at discrete regions of the PVM as does EXP-2. D. REX1(Δ371–579)-GFP-infected RBCs were fixed with paraformaldehyde/ glutaraldehyde and probed with anti-EXP2 (green) and anti-REX1 (red). Samples were examined using deconvolution microscopy. Scale bars = 3 μm; zoom bar = 1 μm.

McHugh E, Batinovic S, Hanssen E, McMillan PJ, Kenny S, Griffin MD, Crawford S, Trenholme KR, Gardiner DL, Dixon MW, Tilley L. A repeat sequence domain of the ring-exported protein-1 of Plasmodium falciparum controls export machinery architecture and virulence protein trafficking. Mol Microbiol. 2015 Aug 24. [Epub ahead of print] PMID:

Other associated proteins

PFID Formal Annotation
PF3D7_0501300 skeleton-binding protein 1
PF3D7_0935900 ring-exported protein 1