Localization of PfAEP in the exonemes of merozoites (A) Co-localization of PfAEP was studied with micronemal resident protein AMA-1 in bursting schizonts. PfAEP (rabbit serum; red) does not co-localize with AMA-1 (green). The staining for PfAEP co-localized with exonemal marker PfSUB1. Scale bar 2 mm. Three-dimensional reconstruction of the confocal z-stack images of schizonts is shown in panel below Scale bar 1.5mm (B) 2D-structured illumination microscopy (SIM) showed co-localization between the staining of PfAEP and PfSUB1 indicating its localization in the exonemes of P. falciparum merozoites. (C) Young rings were immunostained with anti PfAEP antibody and the antibody against MSP119 as a positive control. No staining was observed for PfAEP in young rings whereas MSP119 showed typical disk like staining around the nucleus.
Hans N, Relan U, Dubey N, Gaur D, Chauhan VS. Identification and localization of a Novel Invasin of Plasmodium falciparum. Mol Biochem Parasitol. 2015 Sep 29. [Epub ahead of print]
Other associated proteins
|PF3D7_0507500||subtilisin-like protease 1|
|PF3D7_0930300||merozoite surface protein 1|
|PF3D7_1137200||basal complex transmembrane protein 1|