Expression of PfAEP was analyzed in different stages of the asexual blood-stage life cycle by confocal immuno-fluorescence microscopy using anti-PfAEP mouse serum (green). Parasite nuclei were counterstained with DAPI (blue). No detectable PfAEP staining was observed in ringand trophozoite stages. In schizonts, PfAEP was detected as a punctate staining characteristic of many apical organelle resident proteins. The scale bar indicates 2 mm. In schizonts, the staining of PfAEP was observed in a punctate manner towards the apex of individual merozoites similar to other invasion proteins.
Hans N, Relan U, Dubey N, Gaur D, Chauhan VS. Identification and localization of a Novel Invasin of Plasmodium falciparum. Mol Biochem Parasitol. 2015 Sep 29. [Epub ahead of print] PMID: