(B) Immunofluorescence analysis (IFA) of parental 3D7 and FCB1 schizonts, as well as 3D7 schizonts harboring the constructs in the indicated concentrations of blasticidin. Parasites were probed with MSP1 isoform-specific antibodies. Merged signals include that of the DNA dye 4,6-diamidino-2-phenylindole (DAPI, blue). Scale bar, 5 mm.
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