Subcellular location of PfCyP-19B determined by immunofluorescence. Trophozoite cultures were prepared for immunofluoresence as described in Section 2, using primary rabbit antibodies to PfCyP-19B and mouse antibodies to LDH, secondary FITC-conjugated anti-rabbit IgG and TRITC-conjugated anti-mouse IgG antibodies, and DAPI stain. Two singly infected erythrocytes (small arrows) and a doubly infected erythrocyte (large arrow) can be seen in this representative picture. Panel A: FITC image (PfCyP-19B); Panel B: TRITC image (LDH); Panel C: DAPI image (nuclear stain); Panel D: FITC and TRITC images superimposed; Panel E: FITC, TRITC, and DAPI images superimposed. Experiments using the different fluorophores separately confirmed that there was no significant cross-fluorescence using any of the filter sets. the protein appeared surprisingly to be distributed throughout the cell, as though it were located in the cytosol.
Gavigan CS, Kiely SP, Hirtzlin J, Bell A. Cyclosporin-binding proteins of Plasmodium falciparum. Int J Parasitol. 2003 Aug;33(9):987-96.