Immuno-EM labelling of KAHRP in knob skeletons. A). Thick section (average of 50 slices) from a cryo-tomogram of schizont skeleton labelled with anti-KAHRP antibody 18.2 and 10 nm gold-conjugated secondary antibody. B) The same field of view shown in A, with 70 slices of a 3D model overlaid, describing knob spirals by the first and last turn (green contours), and gold beads as cyan spheres. C) Sections (30 slices each) through individual knobs in a cryo-tomogram of schizont skeleton labelled with mAb 18.2 and gold-conjugated secondary antibody, showing spiral structure with radiating pattern and gold labelling on the coat layer. Connected cytoskeleton is also visible.
Watermeyer JM, Hale VL, Hackett F, Clare DK, Cutts EE, Vakonakis I, Fleck RA, Blackman MJ, Saibil HR. A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes. Blood. 2015 [Epub ahead of print] PMID: