PF3D7_0207600 serine repeat antigen 5

Detection of nitric oxide synthase in both healthy and P. falciparum-harboring red blood cells. NADPH-diaphorase activity within healthy RBCs or PfRBCs at different stages of parasite development (A). The panel (A1) represents the negative control (uRBCs or PfRBCs incubated with NBT without NADPH). NADPHd staining (blue color) was observed in both uRBC and parasite (A2). Identification by immunofluorescence assay (IF) of NOS within uRBCs or PfRBCs using rabbit polyclonal antibody against uNOS (B1, green) or mouse monoclonal antibody against eNOS (B2, green). To differentiate infected from uninfected RBCs, parasite nucleus was labeled in blue with Hoechst 33342. A monoclonal antibody against parasite SERA5 was used to localize the parasitophorous vacuole (red).

Rey J, Buffet PA, Ciceron L, Milon G, Mercereau-Puijalon O, Safeukui I. Reduced erythrocyte deformability associated with hypoargininemia during Plasmodium falciparum malaria. Sci Rep. 2014 Jan 20;4:3767

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