Indirect immunofluorescence assay of recombinant CRDtmWR1 (recombinant SURFIN4.2 proteins containing intracellular region) and CRDtm (recombinant SURFIN4.2 proteins not containing intracellular region). CRDtmWR1 and CRDtm were dual stained with mouse anti-Ty antibody (α-Ty, green; Monoclonal antibody raised in mouse against the Ty1 tag (amino acid sequence EVHTNQDPLD) for recombinant SURFIN4.2 and rabbit anti-PfSBP1 serum (α-SBP1, red) for Maurer’s clefts. Dual staining negative control images with mouse normal IgG (Mo IgG) and anti-PfSBP1 are also shown. Bright field images (BF) are shown, as well as merged images (merge) of α-Ty, α-SBP1, and DAPI (blue) DNA staining. The bar = 2 μm.
Kagaya W, Miyazaki S, Yahata K, Ohta N, Kaneko O. The Cytoplasmic Region of Plasmodium falciparum SURFIN4.2 Is Required for Transport from Maurer's Clefts to the Red Blood Cell Surface. Trop Med Health. 2015 Dec;43(4):265-72.
Other associated proteins
|PF3D7_0424400||surface-associated interspersed protein 4.2 (SURFIN 4.2)|