Localization of PfBlm in different intra-erythrocytic stages of P. falciparum by immunofluorescence staining and confocal microscopy. The cells were fixed and immunostained with purified primary antibodies against PfBlm followed by fluoro-isothiocyanate-labelled secondary antibody and then counterstained with DAPI. In each panel, single confocal image of each stage is shown. (a) Ring stage, (b) Trophozoite stage, (c) Early schizont stage, (d) Schizont stage, (e) Double infection in schizont stage, (f) Bursting schizont stage. In each panel (i) phase contrast image; (ii) image of cell stained with DAPI (blue); (iii) immunofluorescently stained cell (green); (iv) super-imposed image; (v) merged.
Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom omologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 Feb 23. [Epub ahead of print]