PF3D7_1133400 apical membrane antigen 1

PfAMA1 translocation is aberrant in schizonts with PfMOP-knockdown. (a) Schizonts from [+]/[-] Shld1 PfMOP-DD parasites were E64-treated, fixed, probed with anti-PfAMA1 and scored as micronemal (M), partially translocated (PT) or surface (S), representative [+] Shld1 parasite IFAs shown. (c) Synchronized schizont stage (40–44 h) parasites, maintained with 250nM (left panel) or 0 nM (right panel) Shld1, were incubated 6 h in presence of 10 mM E64, methanol-fixed, permeabilized, and stained using antibodies against PfRON4, PfRhopH3, PfEBA175 and PfTubulin. Staining for these markers was similar in [+] and [-] Shld1 conditions. PfAMA1 staining was used to identify E64-treated schizonts that were sufficiently mature (that is, surface staining or partially translocated staining, but not micronemal staining, scale bar, 1 mm).

Absalon S, Robbins JA, Dvorin JD. An essential malaria protein defines the architecture of blood-stage and transmission-stage parasites. Nat Commun. 2016 7:11449.

Other associated proteins

PFID Formal Annotation
PF3D7_0707300 rhoptry-associated membrane antigen
PF3D7_0731500 erythrocyte binding antigen-175
PF3D7_0903700 alpha tubulin 1
PF3D7_0917000 condensin complex subunit 3, putative? cnd3
PF3D7_1116000 rhoptry neck protein 4
PF3D7_1469600 acetyl-coa carboxylase