Indirect immunofluorescence assay on thin smears of schizont and merozoites from wild-type and Δdblmsp parasites showing expression of DBLMSP (green; first panel) and MSP1 (red; second panel). Third panel depicts the overlay of both antibodies with the nuclear stain DAPI; forth panel shows the corresponding brightfield images. Size bars represent 1μm.
Crosnier C, Iqbal Z, Knuepfer E, Maciuca S, Perrin AJ, Kamuyu G, Goulding D, Bustamante LY, Miles A, Moore SC, Dougan G, Holder AA, Kwiatkowski DP, Rayner JC, Pleass RJ, Wright GJ. Binding of Plasmodium falciparum merozoite surface proteins DBLMSP and DBLMSP2 to human immunoglobulin M is conserved amongst broadly diverged sequence variants. J Biol Chem. 2016 May 12. [Epub ahead of print] PMID: