Localization of PfRab5bQ94L-YFP-DD to the TVN. a Time-lapse imaging of PfRab5bQ94L-YFP-DD (green) and TR-ceramide (red) fluorescence after stabilization with Shld1. PfRab5bQ94L-YFP-DD and TR-ceramide was co-localized to a rapidly moving compartment (arrowheads). b The pseudocolors of TR-ceramide at 0, 100, and 200 s were converted to red, green and blue, respectively, and then merged into a single frame. Several extended, mobile TVNs are shown in each color (arrowhead as an example), whereas a stable PVM is shown in white. (c) Cells expressing PfRab5bQ94L-YFP-DD (green) were stained with anti-PfTPx-1 antibody (red) and DAPI (blue). Cytoplasmic PfTPx-1 was not detected from the TVN, where PfRab5bQ94L-YFP-DD (arrowheads) localizes. Bars 5 μmץ

Ebine K, Hirai M, Sakaguchi M, Yahata K, Kaneko O, Saito-Nakano Y. Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular network in

infected red blood cells together with N-acylated adenylate kinase 2. Malar J. 2016 15:323.