PF3D7_1012200 rhoptry associated adhesin

Native protein expression of PfRA in P. falciparum. Immunofluorescence assays were performed on fixed smears of different stages of the P. falciparum intraerythrocytic life cycle. PfRA (green) staining was detected by the anti-PfRA mouse antibodies only in the schizont stages. No signal was observed in trophozoites and schizonts. A distinct punctate staining of PfRA was observed in the schizont which is characteristic of apically localized proteins. Pre-immune antibodies failed to detect any staining. Nuclei were stained with the DNA intercalating dye, DAPI. (Scale bar, 2 μm.)

Anand G, Reddy KS, Pandey AK, Mian SY, Singh H, Mittal SA, Amlabu E, Bassat Q, Mayor A, Chauhan VS, Gaur D. A novel Plasmodium falciparum rhoptry associated adhesin mediates erythrocyte invasion through the sialic-acid dependent pathway. Sci Rep. 2016 6:29185.