PfRH2b is associated with the moving junction during merozoite invasion. The process of merozoite invasion was closely monitored to locate the position of PfRH2b of burst schizont, free merozoites, random merozoite attachment, apposed merozoite and penetrating merozoite. The presence of PfRH2b (PfRH2b, red) at the moving junction was further confirmed with AMA1 (C, green) as common markers with punctuate staining. Merozoites of focus are shown with red circles. The bright field images labeled as Bf. Scale bar indicates 2 μm. (D) A slice
of a three-dimensional structured illumination microscopy (3D-SIM) on merozoite at the junction further confirmed the punctuated pattern of PfRH2b (green) to be in close proximity to AMA1 (red). Scale bar indicates 1 μm. In the merged images, areas of overlap between the red and the green signals are shown in
yellow. DAPI (blue) stained for nuclei.
Aniweh Y, Gao X, Gunalan K, Preiser PR. PfRH2b Specific Monoclonal Antibodies Inhibit Merozoite Invasion. Mol Microbiol. 2016 Jul 20.
Other associated proteins
|PF3D7_1335300||reticulocyte binding protein 2 homologue b, ALKBH5|