Targeted gene disruption of PfPAP changes the filterability of the infected RBCs but not the expression or localization of KAHRP, REX1, and PfEMP-3. B). Immunofluorescence using anti-KAHRP, REX1, and PfEMP-3 antibodies on 3D7 and 3D7_PAP KO show no difference in the location of these proteins. C). Electron microscopy of 3D7_PAP-KO showing electron dense structures at the surface of the infected erythrocyte indicative of the presence of knobs. Bars are 5 mm.

da Silva FL, Dixon MW, Stack CM, Teuscher F, Taran E, Jones MK, Lovas E, Tilley L, Brown CL, Trenholme KR, Dalton JP, Gardiner DL, Skinner-Adams TS. A Plasmodium falciparum S33 proline aminopeptidase is associated with changes in erythrocyte deformability. Exp Parasitol. 2016 Jun 30. pii:S0014-4894(16)30135-7.