Co-localization of PfBlm and PfWrn by immunofluorescence dual colour staining and confocal microscopy. Panels a, b, c, d and e show the images of merozoite attached to RBC, trophozoite, early schizont, mature schizont and late schizont respectively. (i) Immunofluorescently stained cell (green, P. falciparum Wrn protein), (ii) immunofluorescently stained cell (red, PfBlm), (iii) super-imposed images of PfBlm and PfWrn, (iv) super-imposed images. The results reveal that PfWrn was found in nucleus as reported and PfBlm is expressed in nucleus as well as in cytoplasm. The co-localization of PfWrn and PfBlm was observed to some extent in invading merozoite (a) and trophozoite stages.

Rahman F, Tarique M, Tuteja R. Plasmodium falciparum Bloom homologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth. Biochim Biophys Acta. 2016 May;1864(5):594-608