PfRH2b is associated with the moving junction during merozoite invasion. The process of merozoite invasion was closely monitored to locate the position of PfRH2b of burst schizont, free merozoites, random merozoite attachment, apposed merozoite and penetrating merozoite. A. Merozoites were stained for MSP1 (MSP1, green) and PfRH2 (PfRH2, red). PfRH2 apical punctuate staining was observed in the free and attaching merozoites while in invading merozoites two punctuate staining were observed at the anchorage of the moving junction. The presence of PfRH2b (PfRH2b, red) at the moving junction was further confirmed with EBA175 (B, green) and AMA1 (C, green) as common markers with punctuate staining. Merozoites of focus are shown with red circles. The bright field images labelled as Bf. Scale bar indicates 2 lm. D. A slice of a three-dimensional structured illumination microscopy (3D-SIM) on merozoite at the junction further confirmed the punctuated pattern of PfRH2b (green) to be in close proximity to AMA1 (red). Scale bar indicates 1 lm. In the merged images, areas of overlap between the red and the green signals are shown in yellow. DAPI (blue) stained for nuclei.

Aniweh Y, Gao X, Gunalan K, Preiser PR. PfRH2b specific monoclonal antibodies inhibit merozoite invasion. Mol Microbiol. 2016 Nov;102(3):386-404.