Upper pannel: Immunofluorescence microscopy using endogenously derived Pf CK2α–HA. Localization with the anti-HA antibodies (green) confirmed
expression in the nucleus (blue, DAPI) and cytosol of fixed parasites in the trophozoite (T) and schizont (S) stage. Scale bar indicates 2 μm. Enlargements of selected areas are marked with a white square. DIC, differential interference contrast microscopy. PF3D7_1108400. The endogenously tagged protein was localized in the parasite’s cytosol, as well as in the nucleus of trophozoite stage parasites. In the schizont stage of the parasite the dual localization of
endogenously tagged CK2 was less pronounced and appeared predominantly cytosolic. Lowe pannel: Expression of Rh2b–HA was verified by IFA using anti-HA antibodies. Scale bar, 2 μm. DIC, differential interference contrast microscopy. Rh2b–HA is localized at the apical pole of nascent merozoites.
Engelberg K, Paul AS, Prinz B, Kono M, Ching W, Heincke D, Dobner T, Spielmann T, Duraisingh MT, Gilberger TW. Specific phosphorylation of the PfRh2b invasion ligand of Plasmodium falciparum. Biochem J. 2013 Jun 15;452(3):457-66.
Other associated proteins
|PF3D7_1108400||casein kinase 2, alpha subunit|