Representative IFA images show food vacuole morphology in parasites treated with vehicle, CR135, and CR142, individually. Anti-Plasmepsin II polyclonal antibody (rabbit) was diluted 1:1000 and an AlexaFluor 488 conjugated anti-rabbit secondary antibody was diluted 1:350. MitoTracker Red stains active mitochondria. Under drug treatment (CR135 or CR142), the globular structure of the food vacuole of a normal parasite was absent, and the vacuole marker Plasmepsin II distributed among many punctate particles scattered throughout the parasite cytosol.
Ke H, Morrisey JM, Qu S, Chantarasriwong O, Mather MW, Theodorakis EA, Vaidya AB. Caged Garcinia xanthones: a novel chemical scaffold with potent antimalarial activity. Antimicrob Agents Chemother. 2016 Oct 31. pii: AAC.01220-16PMID: