Representative IFA images show food vacuole morphology in parasites treated with vehicle, CR135, and CR142, individually. Anti-Plasmepsin II polyclonal antibody (rabbit) was diluted 1:1000 and an AlexaFluor 488 conjugated anti-rabbit secondary antibody was diluted 1:350. MitoTracker Red stains active mitochondria. Under drug treatment (CR135 or CR142), the globular structure of the food vacuole of a normal parasite was absent, and the vacuole marker Plasmepsin II distributed among many punctate particles scattered throughout the parasite cytosol.

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