RhopH2 and RhopH3 are membrane-associated proteins. IFA of indicated proteins in R2glmS (RopH2 knockdown) and R3glmS (RopH3 knockdown) at the trophozoite stage. IFA of mature schizont-infected cells with indicated antibodies. Punctate labeling indicates that these proteins localize to rhoptry organelles in daughter merozoites. Scale bar, Scale bars, 5 μm. IFA of trophozoite-infected cells, showing that each protein localizes to the host membrane and to the vacuole surrounding the intracellular parasite. Scale bar, 5 μmImmunofluorescence microscopy confirmed packaging into rhoptries within schizont-infected cells (B). Imaging of maturing trophozoite-stage parasites also showed that these proteins traffic to the host membrane (C). Because each gene is transcribed and translated only within schizonts, the protein found in these maturing trophozoites reflects transfer from the merozoite during invasion.

Ito D, Schureck MA, Desai SA. An essential dual-function complex mediates erythrocyte invasion and channel-mediated nutrient uptake in malaria parasites. Elife. 2017 Feb 21;6.