Truncation of RhopH3 leads to mistrafficking of components of the

RhopH complex and loss of complex formation. Mislocalisation and reduced levels of RhopH3 in naturally released free merozoites of rhopH3-loxP parasites treated with rapamycin. Samples were probed with a monoclonal antibody to the merozoite surface marker MSP1 as well as anti-RhopH3 antibodies. Scale bar, 2 μm. IFA of naturally released free merozoites showed that the truncated RhopH3Δ4-6 was often largely undetectable in merozoites of the mutant Parasites.

Sherling ES, Knuepfer E, Brzostowski JA, Miller LH, Blackman MJ, van Ooij C. The Plasmodium falciparum rhoptry protein RhopH3 plays essential roles in host cell invasion and nutrient uptake. Elife. 2017 6. pii: e23239.