Generation of transgenic parasites in which RhopH2 is epitope-tagged. Immunofluorescence analysis (IFA) on schizonts fixed with acetone/methanol and labelled with anti-HA antibody to detect RhopH2 and other antibodies, as indicated. Immunofluorescence analysis (IFA) confirmed RhopH2-HA localized to the rhoptry and co-localized with other rhoptry bulb proteins, RhopH1, RhopH3 and RAMA but not with the rhoptry neck protein, RON4, the micronemal marker, AMA-1 or the plasma membrane protein MSP1.
Counihan N, Chisholm SA, Bullen HE, Srivastava A, Sanders PR, Jonsdottir TK, Weiss GE, Ghosh S, Crabb BS, Creek DJ, Gilson PR, de Koning-Ward TF. Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate. Elife. 2017 Mar 2;6. pii: e23217
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0707300 | rhoptry-associated membrane antigen |
PF3D7_0905400 | high molecular weight rhoptry protein 3 |
PF3D7_0929400 | high molecular weight rhoptry protein 2 |
PF3D7_0930300 | merozoite surface protein 1 |
PF3D7_1116000 | rhoptry neck protein 4 |
PF3D7_1133400 | apical membrane antigen 1 |