Localization and KS with protein targets. (a,b) Knockin cell lines of CDPK5-2×FKBP-GFP with an mCherry-tagged nuclear mislocalizer (MislocalizerN) (a) and HP1-2×FKBP-GFP with a plasma-membrane mislocalizer (MislocalizerP). In synchronized parasites, KS of CDPK5 caused an accumulation of segmented schizonts. (b). Representative fluorescence microscopy images of rapalog-treated and control cells (after 16 h). Mislocalization of HP1 caused an accumulation of gametocytes.

Birnbaum J, Flemming S, Reichard N, Soares AB, Mesén-Ramírez P, Jonscher E, Bergmann B, Spielmann T. A genetic system to study Plasmodium falciparum protein function. Nat Methods. 2017 Mar 13.