PfPKG inhibitors block discharge of exonemes and micronemes. (A) IFA of wt 3D7 parasites allowed to develop beyond the point of egress in the presence of E64 only (50 mM). Top two rows: segmented schizonts displaying a merozoite surface localisation of PfAMA1 (arrowed; ,45% of the schizont population) as a result of its discharge from micronemes, exhibit a weak PfSUB1 signal compared to less mature schizonts in which both the PfAMA1 and PfSUB1 signals remain punctate. The bottom row of images in (A) shows a schizont in which the PfAMA1 signal is intermediate between punctate and merozoite surface. (C) IFA of wt 3D7 parasites allowed to develop beyond the point of egress in the presence of both E64 and C1. No discharge or relocalisation of PfSUB1 or PfAMA1 was evident in counts of .5,000 schizonts from a total of 3 independent experiments. Identical results were obtained with E64 plus C2 (not shown).
Collins CR, Hackett F, Strath M, Penzo M, Withers-Martinez C, Baker DA, Blackman MJ. Malaria parasite cGMP-dependent protein kinase regulates blood stage merozoite secretory organelle discharge and egress. PLoS Pathog. 2013 9(5):e1003344.
Other associated proteins
|PF3D7_0507500||subtilisin-like protease 1|