Immunofluorescence images showing PfTPxGl and PfFC (mitochondrial marker protein) trafficking in AlF4−, nocodazole and vineblsatine treated 3D7 parasites

(A) PfTPxGl and PfFC co-localization in AlF4−-treated parasites, (B) PfTPxGl and PfFC co-localization in vinblastine-treated parasites, (C) PfTPxGl and PfFC co-localization in nocodazole-treated parasites. In this experiment, PfTPxGl was found to be co-localized with the mitochondrial marker protein PfFC in 40% of the treated parasites suggesting that trafficking of PfTPxGl to the mitochondrion may be partially disrupted by the treatments. Scale Bar: 10 µm. When the parasites from the same treated cultures were analyzed for co-localization of the disrupted PfTPxGl signal with mitochondrial marker protein ferrochelatase (PfFC), we observed that in 35-40% of cells the PfTPxGl signal showed some overlap.

Chaudhari R, Dey V, Narayan A, Sharma S, Patankar S. Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum. PeerJ. 2017 Apr 27;5:e3128. PMID: 28462015