PF3D7_1212000 glutathione peroxidase-like thioredoxin peroxidase

PfTPxGl localization to the outermost membrane of the apicoplast in D10-ACPleader-GFP parasites. (C) Localization of PfTPxGl to the membranes of intact/permeabilized apicoplasts from control parasites, (D) Absence of PfTPxGl in the membranes of intact/permeabilized apicoplasts from AlF4- treated parasites. Scale Bars as indicated in the figures. anti-GFP antibodies stained the apicoplast lumen (red signal) only in the permeabilized organelles, but not in the intact ones (C). This immunofluorescence signal colocalized with the intrinsic GFP fluorescence (green signal) of ACP-GFP. In both fractions, anti-PfTPxGl antibodies clearly showed staining surrounding the fluorescent signals from luminal ACP-GFP. While the size and morphology of free intact apicoplasts (based on ACP-GFP staining) remained unaltered, no peri-organellar PfTPxGl signal was observed in the immunofluorescence analysis of intact as well as permeabilized organelles, indicating the involvement of vesicles in the membrane targeting of this protein (D).

Chaudhari R, Dey V, Narayan A, Sharma S, Patankar S. Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum. PeerJ. 2017 Apr 27;5:e3128.

Other associated proteins

PFID Formal Annotation
PF3D7_0208500 acyl carrier protein
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