Induction of PfSR1 expression using the DDD system. Immuno-fluorescence assay (IFA) of PfSR1-GFP-DDD expressing parasites growing on 5 μM TMP showing the cellular localization of the induced PfSR1-GFP throughout the IDC. Tagged PfSR1 was detected using anti-GFP antibody. PfSR1 is shown in green; nuclei were stained with DAPI (blue). in early ring stages PfSR1-GFP-DDD localizes to the nucleus in foci that are mainly located at the nuclear periphery. This pattern changes later in the IDC at the trophozoite and schizont stages, when PfSR1-GFP-DDD can be detected in both the nucleus and in the cytoplasm .

Eshar S, Altenhofen L, Rabner A, Ross P, Fastman Y, Mandel-Gutfreund Y, Karni R, Llinás M, Dzikowski R. PfSR1 controls alternative splicing and steady-state RNA levels in Plasmodium falciparum through preferential recognition of specific RNA motifs. Mol Microbiol. 2015 Jun;96(6):1283-97.

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