Immunolocalization of P. falciparum LDH and GAPDH in asexual P. falciparum (3D7) parasites grown in vitro, synchronized by sorbitol treatment and prepared at ring, trophozoite and schizont stages as indicated alongside the panels. Fixed parasites were probed with chicken anti-rPfLDH or anti-rPfGAPDH antibodies and the chicken antibodies were detected with a Cy3-conjugated antibody. Parasite DNA was stained with Hoechst and the scale bar represents 5 μm. DIC images are shown as reference. PfGAPDH and PfLDH showed very similar 442 distribution throughout the cytoplasm of the parasites, however PfGAPDH seemed to localize to the nucleus in schizont stages.
Krause RGE, Hurdayal R, Choveaux D, Przyborski J, Coetzer THT, Goldring JPD. Plasmodium glyceraldehyde-3-phosphate dehydrogenase: A potential malaria diagnostic target. Exp Parasitol. 2017 May 25. [Epub ahead of print] PMID: 28552792
Other associated proteins