IFA of parasites grown in the presence and absence of Shld probed with antibodies against PfRON4 (green) and PfGAP45 (red). Bars, 2 mm. Staining for PfGAP45, a soluble protein that complexes with PfGAP50 (along with PfMyoA and PfMTIP) to localize to the inner membrane complex (IMC), revealed readily detected expression of the PfGAP45 protein but the absence of normal morphology in PfCyc1-deficient parasites. In contrast, IFAs against the rhoptry protein PfRON4 suggests unencumbered rhoptry formation. Similarly, IFA against the In contrast, IFAs against the rhoptry protein PfRON4 suggests unencumbered rhoptry formation.

Robbins JA, Absalon S, Streva VA, Dvorin JD. The Malaria Parasite Cyclin H Homolog PfCyc1 Is Required for Efficient Cytokinesis in Blood-Stage Plasmodium falciparum. MBio. 2017 Jun 13;8(3). pii: e00605-17.