Endogenous PfORC1 binds to PfARS sequences in Plasmodium falciparum. FISH-IFA was performed using fluorescence labeled ARS3 DNA probe and antibodies against ORC1 in the trophozoite stage (25-30hpi) parasites. Results indicate ~75% co-localization of PfARS3 signals with ORC1.Right panel shows the graphical representation of merged signals versus apart signals. (E) FISH-IFA experiments were performed using non-ARS sequence as a probe. Co-localization of non-ARS signals with ORC1was rare (<10%). Right panel shows the graphical representation of co-localized signals versus non-colocalized signals for twenty images in each case.
Agarwal M, Bhowmick K, Shah K, Krishnamachari A, Dhar SK. Identification and Characterization of ARS like sequences as putative origin(s) of replication in human malaria parasite Plasmodium falciparum. FEBS J. 2017 Jun 23. [Epub ahead of print]