Location and protease accessibility of PfEMP1B during export. Immunofluorescence analysis of PfEMP1B infected RBCs labelled with antisera recognizing GFP (green) and V5 or EXP2 or MAHRP1 (red). The GFP and V5 epitope tags on PfEMP1B are recognized in transgenic parasites expressing the chimera in a compartment with a ‘necklace of beads’ profile at the parasite periphery. Differential interference contrast (DIC) image and parasite nuclei (stained with DAPI; blue) are shown on the left. White arrows indicate PV-localized PfEMP1B while blue arrows indicate PfEMP1B located at the Maurer’s clefts. Scale bars, 5 mm. These compartments respectively represent the PV (where there is partial co-localization with PTEX component exported protein-2 (EXP2)) and the Maurer’s clefts (co-localization with Maurer’s cleft protein membrane-associated histidine-rich protein-1 (MAHRP1)).
Batinovic S, McHugh E, Chisholm SA, Matthews K, Liu B, Dumont L, Charnaud SC, Schneider MP, Gilson PR, de Koning-Ward TF, Dixon MWA, Tilley L. An exported protein-interacting complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes. Nat Commun. 2017 10;8:16044.
Other associated proteins