DiCre-mediated conditional disruption of P. falciparum SERA5 expression. IFA of mature schizonts of control (DMSO-treated) and RAP-treated integrant clone floxSERA5-1B6 ~44 h following treatment. No SERA5-specific signal was detectable in the majority (~98%) of the RAP-treated population. Scale bar, 5 μm. To assess the effects of SERA5 gene deletion on SERA5 protein expression, mature flox-SERA5-1B6 and floxSERA5-3B6 schizonts were examined by indirect immunofluorescence (IFA) at the end of the first erythrocytic cycle (~44 h) following RAP-treatment. No SERA5-specific signal was detectable by IFA in most of the RAP-treated floxSERA5-1B6 and flox-SERA5-3B6 schizonts

Collins CR, Hackett F, Atid J, Tan MSY, Blackman MJ. The Plasmodium falciparum pseudoprotease SERA5 regulates the kinetics and efficiency of malaria parasite egress from host erythrocytes. PLoS Pathog. 2017 Jul 6;13(7):e1006453.