Live cell microscopy images of the endogenously GFP-tagged candidate PF3D7_0629200 (PFF1415c DnaJ protein) show PV location. The top microscopy panel row shows a ring and a trophozoite stage, the middle row shows a schizont and the bottom row shows a late schizont. The protein is not expressed in ring stages but in trophozoites and showed a signal in the parasite periphery consistent with a PV location. In addition pronounced foci in close proximity of the nucleus were

detected, suggesting some protein remaining in the secretory pathway. It is therefore likely a protein found within the parasite and peripherally attached at the outer face of the PPM or the inner face of the PVM of trophozoites

Khosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T. Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID. Int J Med Microbiol. 2017 Jul 27. [Epub ahead of print]