IFA shows a loss of reactivity of PfACT1 KOs to the anti-PfACT1 antibody. The PV is depicted in red (Sera-5). Scale bar 5 μ. PfACT1 disruption was apparent by immunofluorescence assay (IFA) in schizonts 44 h post-induction. Based on IFAs, it was estimated that ~98% of the population had undergone excision of pfact1, resulting in an almost pure population of pfact1 disrupted parasites (PfACT1 KO) for phenotypic analysis. Only a weak, potentially non-specific signal could be detected by IFA in PfACT1 KO schizonts.
Das S, Lemgruber L, Tay CL, Baum J, Meissner M. Multiple essential functions of Plasmodium falciparum actin-1 during malaria blood-stage development. BMC Biol. 2017 Aug 15;15(1):70.
PubMed Article: Multiple essential functions of Plasmodium falciparum actin-1 during malaria blood-stage development
Other associated proteins
|PF3D7_0207600||serine repeat antigen 5|