PfELC expression matches that of known glideosome components in P. falciparum schizonts. Immunofluorescent detection of HA-tagged ELC and either MTIP or GAP45 in mature schizonts and merozoites. Merged colour images are also shown, with ELC-HA (green) and MTIP or GAP45 (red). Nuclei are stained with DAPI (blue). Scale bar: 2 μm. Immunofluorescence analysis of fixed blood-stage P. falciparum with HA-specific antibodies showed co-localisation of the protein with GAP45 and MTIP in late, segmented schizonts.
Green JL, Wall RJ, Vahokoski J, Yusuf NA, Ridzuan MAM, Stanway RR, Stock J, Knuepfer E, Brady D, Martin SR, Howell SA, Pires IP, Moon RW, Molloy JE, Kursula I, Tewari R, Holder AA. Compositional and expression analyses of the glideosome during the Plasmodium life cycle reveal an additional myosin light chain required for maximum motility. J Biol Chem. 2017 Sep 11.
Other associated proteins
|PF3D7_1017500||myosin essential light chain ELC|
|PF3D7_1222700||glideosome-associated protein 45|