CQ responses of CQS parasites (strain HB3) transfected with a minimal mutant of PfCRTDd2 or PfCRTEcu1110 (D28 and E2, respectively). (The HB3, Dd2, D28, and E2 versions of PfCRT were expressed episomally as GFP-tagged proteins in the CQS P. falciparum strain HB3. In each case, live-cell imaging revealed that fluorescence was restricted to the region surrounding the hemozoin crystals, consistent with PfCRT-GFP localizing to the DV [note that PfCRT has been shown previously to localize to the parasite’s DV (9, 18)]. (Scale bars: 2 μm.)

Summers RL, Dave A, Dolstra TJ, Bellanca S, Marchetti RV, Nash MN, Richards SN, Goh V, Schenk RL, Stein WD, Kirk K, Sanchez CP, Lanzer M, Martin RE. Diverse mutational pathways converge on saturable chloroquine transport via the malaria parasite's chloroquine resistance transporter. Proc Natl Acad Sci U S A. 2014 111(17):E1759-67. PMID: 24728833