Dual labeling of PfShelph2-HA3 with rhoptry neck and exoneme markers. IFA of PfShelph2-HA3 parasites using anti-HA and (A) anti-PfRON4 or (B) anti-PfSUB1 antibodies on schizonts. Scale bar, 2 μm. no obvious overlap between PfShelph2 and PfRON4 signals in schizonts (A). PfSUB1 is discharged in the parasitophorous

vacuole just prior egress, and therefore, exonemes are considered as secretory organelles. A very distinct pattern between PfSUB1 and PfShelph2-HA3 is observed indicating that PfShelph2 is not contained within exonemes. PfShelph2 displays an expression profile similar to many invasion-related proteins, and is located in an apical compartment in the invasive merozoite that is different from the rhotry neck or exonemes.

Miliu A, Lebrun M, Braun-Breton C, Lamarque MH. Shelph2, a bacterial-like phosphatase of the malaria parasite Plasmodium falciparum, is dispensable during asexual blood stage. PLoS One. 2017 Oct 26;12(10):e0187073