Immunofluorescence analysis of histone modifications in P. falciparum. (b) Immunofluorescence analysis of H4K31ac (in red) in asexual stages following 12 hours of treatment with DMSO (vehicle) or FR235222 HDAC inhibitor. Parasite nuclear DNA was stained with Hoechst (blue). Scale bar, 10 mm. We observed an apparent non-overlapping staining for acetylated and methylated H4K31 and more specifically a discrete focal distribution of H4K31me1 at the nuclear periphery.

Sindikubwabo F, Ding S, Hussain T, Ortet P, Barakat M, Baumgarten S, Cannella D, Palencia A, Bougdour A, Belmudes L, Couté Y, Tardieux I, Botté CY, Scherf A, Hakimi MA. Modifications at K31 on the lateral surface of histone H4 contribute to genome structure and expression in apicomplexan parasites. Elife. 2017 Nov 4;6. pii: e29391.