Localization and signal intensity comparison of mini-SURFIN4.1, NTC-GFP and NTC-4.2WRD2-GFP, in P. falciparum-iRBC. a Co-localization of NTC-GFP with the PVM marker EXP2 and Maurer’s cleft marker SBP1. Upper panel Schematic drawing of NTC-GFP. Lower panels Representative fluorescence images showing the co-localization of NTC-GFP with EXP2 and SBP1. b Co-localization of NTC-4.2WRD2-GFP with EXP2, SBP1 and PfEMP1. Upper panel Schematic drawing of NTC-4.2WRD2-GFP. Lower panels Representative fluorescence images showing the co-localization of NTC-4.2WRD2-GFP with EXP2, SBP1 and PfEMP1. The differential interference contrast images merged with nucleus stained with DAPI (DIC + Nuc), fluorescence images with mouse anti-GFP antibody (anti-GFP), PVM location with rabbit anti-EXP2 antibody (anti-EXP2) or Maurer’s cleft location with rabbit anti-SBP1 antibody (anti-SBP1), or rat anti-PfEMP1 antibody (anti-PfEMP1), and merged image are shown. Bar 5 μm. IFA with the PVM marker EXP2 revealed that NTC-GFP was exported beyond the PVM into infected RBC (iRBC) cytosol, and the signals showed a dotted pattern, which co-localized with the Maurer’s cleft marker SBP1, suggesting Maurer’s cleft localization (a). The dominant fluorescence signals were detected in parasite cytosol and Maurer’s cleft. However, NTC-4.2WRD2-GFP produced signals beyond the PVM with a diffused localization pattern in the iRBCs, which only partially co-localized with the Maurer’s cleft marker SBP1 and with PfEMP1, suggesting that it was likely transported beyond Maurer’s clefts and to the RBC cytosol or membrane (b).
Zhu X, He Y, Liang Y, Kaneko O, Cui L, Cao Y. Tryptophan-rich domains of Plasmodium falciparum SURFIN(4.2) and Plasmodium vivax PvSTP2 interact with membrane skeleton of red blood cell. Malar J. 2017 16(1):121. PMID: 28320404ì
Other associated proteins
|PF3D7_0424400||surface-associated interspersed protein 4.2 (SURFIN 4.2)|
|PF3D7_1471100||exported protein 2|