PF3D7_1370300 membrane associated histidine-rich protein

PfEMP1 trafficking and surface exposure on KAHRP mutant cell lines. (A) Immunofluorescence assay on 3D7-K119– and 3D7-K(re)–infected erythrocytes with

anti-ATS antibody detecting PfEMP1 and anti-MAHRP, a Maurer clefts marker. The first panel in each row shows a phase-contrast image, the second panel is nuclear DNA stained with DAPI, the third panel is the reaction with the anti-ATS antibody for PfEMP1 detection, the fourth panel is the reaction with anti-MAHRP antibody, and the fifth panel illustrates an overlay of the previous 4 panels with colocalization shown in yellow. Parasite-associated PfEMP1 labeling was detected in both cell lines and in the erythrocyte the protein was localized to Maurer clefts as demonstrated by colocalization with MAHRP. both transfectants displayed rim fluorescence indicating that trafficking of PfEMP1 to Maurer clefts and the erythrocyte membrane was not impaired in transgenic cells.

Rug M, Prescott SW, Fernandez KM, Cooke BM, Cowman AF. The role of KAHRP domains in knob formation and cytoadherence of P falciparum-infected human erythrocytes. Blood. 2006 108(1):370-8.

Other associated proteins

PFID Formal Annotation