Expression analysis and co-localization of PF11_0503 with PfSBP-1 and PfEMP-1 at different stages of asexual parasite life cycle. Smears of methanol fixed Pf3D7-infected erythrocytes were stained with anti-PHIST antibodies (1:500), anti PfSBP-1 antibodies (1:1000) and anti PfEMP-1 antibodies (1:500), followed by incubation with Alexa flour-conjugated secondary antibodies (Alexa fluor 488 – green and Alexa fluor 546 – red). DIC: differential interference contrast image, DAPI: nuclear staining using DAPI (blue), PfEMP-1: mice anti-ATS antibody (red), PfSBP-1: anti-PfSBP-1 antibody (red), PF11_0503 : rabbit anti-PF11_0503 antibodies (green), Merge: overlay of PHIST proteins with PfEMP-1 or PfSBP-1. PF11_0503 showed a similar expression pattern as that of PfSBP-1 at the ring stage of parasite development (C). At mature trophozoite and early schizont stages, PF11_0503 showed limited overlap with PfSBP-1 as punctuate foci, while a more homogenous distribution around the periphery of iRBCs surrounding PfSBP-1 fluorescence could be seen at mature schizont stage. This PHIST protein had a very confined and remarkable overlapping with PfEMP-1 in mature schizonts, suggesting iRBC membrane to be their final destination.
Kumar V, Kaur J, Singh AP, Singh V, Bisht A, Panda JJ, Mishra PC, Hora R.PHISTc protein family members localize to different sub-cellular organelles and bind Plasmodium falciparum major virulence factor 'PfEMP-1'. FEBS J. 2017 Nov 20.
Other associated proteins
|PF3D7_0501300||skeleton-binding protein 1|