PF3D7_0208500 acyl carrier protein

Right panel: ATG8 depletion leads to terminal apicoplast loss. Representative microscopy images showing localization of apicoplast-targeted GFP (ACPL-GFP), in schizont stage ATG8+ or ATG8-deficient/IPP-rescued parasites depleted of ATG8 for 2 replication cycles. Bar, 5 mm. ATG8 knockdown leads to defects in apicoplast inheritance. (A) Representative images of apicoplast FISH detecting the apicoplast genome in ring-stage ATG8 and ATG8-deficient parasites 48 h after aTC removal, i.e., after first reinvasion (top and middle rows, respectively). Apicoplast-minus parasites, generated by 4 cycles of chloramphenicol treatment and IPP rescue, are shown as a negative control for FISH staining (bottom row). (C) Representative images of ring-stage ATG8+ or ATG8-deficient parasites expressing ACPL-GFP 48 h after aTC removal, i.e., after first reinvasion. Bars, 5 mm.

Walczak M, Ganesan SM, Niles JC, Yeh E. ATG8 Is Essential Specifically for an Autophagy-Independent Function in Apicoplast Biogenesis in Blood-Stage Malaria Parasites. MBio. 2018 Jan 2;9(1). pii: e02021-17. PMID: 29295911

Other associated proteins

PFID Formal Annotation
PF3D7_1019900 autophagy-related protein 8
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