Plasmodium falciparum sortilin localizes to the cis region of the Golgi apparatus. (A) IFA showing extensive colocalization between PfSortilin-3HA and the cis-Golgi marker ERD2. Some overlap is also seen with the trans-Golgi marker GFP-Rab6, while little overlap is obtained with the late endosome marker GFP-Rab7 (C) IFA showing that PfSortilin-3HA does not colocalize with either the micronemal marker AMA1 or the rhoptry markers RAP1 and RAMA. Extensive colocalization
with markers of the cis-Golgi (endoplasmic reticulum retention-defective
ERD2) and the trans-Golgi (Rab6) confirmed that PfSortilin was indeed localized to the Golgi (Ai and ii). Toxoplasma gondii sortilin (TgSortilin) partially colocalizes with the endosome-like compartments, we consequently determined whether the same was true in P. falciparum by colocalization with Rab7, a marker of the late endosome. Our results showed that despite being very close, the two signals never overlapped in schizont stages (2Aiii and C). This suggests that the major proportion of PfSortilin is found at the cis-Golgi apparatus in schizont-stage parasites.
Hallée S, Boddey JA, Cowman AF, Richard D. Evidence that the Plasmodium falciparum Protein Sortilin Potentially Acts as an Escorter for the Trafficking of the Rhoptry-Associated Membrane Antigen to the Rhoptries. mSphere. 2018 3(1). pii: e00551-17.
Other associated proteins
|PF3D7_0707300||rhoptry-associated membrane antigen|
|PF3D7_1133400||apical membrane antigen 1|
|PF3D7_1144900||ras-related protein Rab-6|
|PF3D7_1353600||ER lumen protein retaining receptor|
|PF3D7_1410400||rhoptry-associated protein 1|