Generation of the 3HA-tagged PfSortilin line. (D) IFA showing that PfSortilin-3HA is expressed throughout the erythrocytic cycle and partially overlaps with the ER marker BiP in rings and trophozoites. (E) IFA showing extensive colocalization between PfSortilin-3HA and the cis-Golgi marker ERD2 at all stages. (F) IFA showing extensive colocalization between PfSortilin-3HA and the trans-Golgi marker GFP-Rab6 at all stages. In ring stages, we could clearly see a distinct focus of fluorescence, along with a perinuclear signal (Di). This pattern was also seen in trophozoites, and some partial colocalization with an anti-binding immunoglobulin protein (anti-BiP) antibody demonstrated that a portion of the protein was found in the endoplasmic reticulum (ER) (Di and ii). In schizont-stage parasites, the fluorescence focus had multiplied and no longer colocalized with BiP, reminiscent of the behavior of other proteins present in the Golgi apparatus during schizogony (Diii). Extensive colocalization with markers of the cis-Golgi (endoplasmic reticulum retention-defective ERD2) and the trans-Golgi (Rab6) confirmed that PfSortilin was indeed localized to the Golgi apparatus as previously shown.
Hallée S, Boddey JA, Cowman AF, Richard D. Evidence that the Plasmodium falciparum Protein Sortilin Potentially Acts as an Escorter for the Trafficking of the Rhoptry-Associated Membrane Antigen to the Rhoptries. mSphere. 2018 3(1). pii: e00551-17.
Other associated proteins
|PF3D7_1144900||ras-related protein Rab-6|
|PF3D7_1353600||ER lumen protein retaining receptor|